《植物生理学报》 2016, 52(5): 685-692

白桦BpUVR8基因的序列与表达模式分析

李晓一, 詹亚光, 娄晓瑞, 曾凡锁^*

东北林业大学生命科学学院, 哈尔滨150040

通信作者：曾凡锁；E-mail: zengfansuo@126.com

摘　要：

本研究克隆了白桦(Betula platyphylla) UVR8基因, 命名为BpUVR8 (GenBank: AHY02156)。生物信息学分析表明该基因全长1 326 bp, 编码441个氨基酸, 为稳定性蛋白, 不存在信号肽, 具有2个跨膜区。二级结构分析表明该蛋白属于混合型。三级结构预测结果显示, BpUVR8与AtUVR8蛋白结构具有较高相似性, 均由7个β螺旋组成片层结构。BpUVR8表达模式分析表明, 其主要在白桦叶片中表达, 且8月份表达量最高; 紫外诱导6 h, 其转录表达水平上调约2倍; 同时, 非生物胁迫以及信号诱导在一定时间内也能影响该基因的转录表达。本文为研究白桦对UV-B信号的响应以及对UVR8功能的深入研究提供参考。

关键词：白桦; UV-B; UVR8; 生物信息学分析

收稿：2015-12-07   修定：2016-04-17

资助：中央高校基本科研业务费专项资金(2572014DA04)、国家自然科学基金(31200463)和黑龙江省博士后启动基金(LBH-Q12166)。

The sequence and expression analysis of BpUVR8 gene in birch

LI Xiao-Yi, Zhan Ya-Guang, LOU Xiao-Rui, Zeng Fan-Suo^*

College of Life Science, Northeast Forestry University, Harbin 150040, China

Corresponding author: Zeng Fan-Suo; E-mail: zengfansuo@126.com

Abstract:

As a photoreceptor specifically for UV-B light, BpUVR8 gene plays an important role in the photomorphogenesis and growing development in plants. We cloned the UVR8 gene in birch, and named it as BpUVR8 (GeneBank ID: AHY02156). The bioinformatics analysis shows that this sequence consists of 1 326 bp with a complete open reading frame, and encodes 441 amino acids. Furthermore, it is a stable hydrophobic protein. This protein may not contain signal peptide but have the transmembrane ability. The protein has two transmembrane domains. According to the tertiary structure prediction, the protein is constituted by a seven-bladed β-propeller, which has a high structural homology with AtUVR8 in Arabidopsis thaliana. The expression analysis of BpUVR8 indicates that this gene expressed in various tissues, and the expression level in leaves is higher than others. The expression of BpUVR8 in August is highest. Besides, this gene is not sensitive to the induction of UV-B signal. Abiotic stress can induce the expression of BpUVR8 gene. This paper can provide a reference for the study of gene function in response to the UV-B signals.

Key words: Betula platyphylla; UV-B; UVR8; bioinformatics analysis